Lignin and veratryl alcohol are not inducers of the ligninolytic system of Phanerochaete chrysosporium.
Identifieur interne : 000E08 ( Main/Exploration ); précédent : 000E07; suivant : 000E09Lignin and veratryl alcohol are not inducers of the ligninolytic system of Phanerochaete chrysosporium.
Auteurs : A M Cancel [États-Unis] ; A B Orth ; M. TienSource :
- Applied and environmental microbiology [ 0099-2240 ] ; 1993.
Descripteurs français
- KwdFr :
- ARN fongique (métabolisme), ARN messager (métabolisme), ARN ribosomique 16S (métabolisme), Alcools benzyliques (pharmacologie), Basidiomycota (effets des médicaments et des substances chimiques), Basidiomycota (métabolisme), Endopeptidases (métabolisme), Induction enzymatique (effets des médicaments et des substances chimiques), Isoenzymes (métabolisme), Lignine (métabolisme), Lignine (pharmacologie), Peroxidases (métabolisme), Stabilité enzymatique (effets des médicaments et des substances chimiques).
- MESH :
- effets des médicaments et des substances chimiques : Basidiomycota, Induction enzymatique, Stabilité enzymatique.
- métabolisme : ARN fongique, ARN messager, ARN ribosomique 16S, Basidiomycota, Endopeptidases, Isoenzymes, Lignine, Peroxidases.
- pharmacologie : Alcools benzyliques, Lignine.
English descriptors
- KwdEn :
- Basidiomycota (drug effects), Basidiomycota (metabolism), Benzyl Alcohols (pharmacology), Endopeptidases (metabolism), Enzyme Induction (drug effects), Enzyme Stability (drug effects), Isoenzymes (metabolism), Lignin (metabolism), Lignin (pharmacology), Peroxidases (metabolism), RNA, Fungal (metabolism), RNA, Messenger (metabolism), RNA, Ribosomal, 16S (metabolism).
- MESH :
- chemical , metabolism : Endopeptidases, Isoenzymes, Lignin, Peroxidases, RNA, Fungal, RNA, Messenger, RNA, Ribosomal, 16S.
- chemical , pharmacology : Benzyl Alcohols, Lignin.
- drug effects : Basidiomycota, Enzyme Induction, Enzyme Stability.
- metabolism : Basidiomycota.
Abstract
Phanerochaete chrysosporium is a white rot fungus which secretes a family of lignin-degrading enzymes under nutrient limitation. In this work, we investigated the roles of veratryl alcohol and lignin in the ligninolytic system of P. chrysosporium BKM-F-1767 cultures grown under nitrogen-limited conditions. Cultures supplemented with 0.4 to 2 mM veratryl alcohol showed increased lignin peroxidase activity. Addition of veratryl alcohol had no effect on Mn-dependent peroxidase activity and inhibited glyoxal oxidase activity. Azure-casein analysis of acidic proteases in the extracellular fluid showed that protease activity decreased during the early stages of secondary metabolism while lignin peroxidase activity was at its peak, suggesting that proteolysis was not involved in the regulation of lignin peroxidase activity during early secondary metabolism. In cultures supplemented with lignin or veratryl alcohol, no induction of mRNA coding for lignin peroxidase H2 or H8 was observed. Veratryl alcohol protected lignin peroxidase isozymes H2 and H8 from inactivation by H2O2. We conclude that veratryl alcohol acts as a stabilizer of lignin peroxidase activity and not as an inducer of lignin peroxidase synthesis.
DOI: 10.1128/AEM.59.9.2909-2913.1993
PubMed: 8215363
PubMed Central: PMC182385
Affiliations:
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Le document en format XML
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Tien</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="ISSN">0099-2240</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (drug effects)</term><term>Basidiomycota (metabolism)</term><term>Benzyl Alcohols (pharmacology)</term><term>Endopeptidases (metabolism)</term><term>Enzyme Induction (drug effects)</term><term>Enzyme Stability (drug effects)</term><term>Isoenzymes (metabolism)</term><term>Lignin (metabolism)</term><term>Lignin (pharmacology)</term><term>Peroxidases (metabolism)</term><term>RNA, Fungal (metabolism)</term><term>RNA, Messenger (metabolism)</term><term>RNA, Ribosomal, 16S (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN fongique (métabolisme)</term><term>ARN messager (métabolisme)</term><term>ARN ribosomique 16S (métabolisme)</term><term>Alcools benzyliques (pharmacologie)</term><term>Basidiomycota (effets des médicaments et des substances chimiques)</term><term>Basidiomycota (métabolisme)</term><term>Endopeptidases (métabolisme)</term><term>Induction enzymatique (effets des médicaments et des substances chimiques)</term><term>Isoenzymes (métabolisme)</term><term>Lignine (métabolisme)</term><term>Lignine (pharmacologie)</term><term>Peroxidases (métabolisme)</term><term>Stabilité enzymatique (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Endopeptidases</term><term>Isoenzymes</term><term>Lignin</term><term>Peroxidases</term><term>RNA, Fungal</term><term>RNA, Messenger</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Benzyl Alcohols</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Basidiomycota</term><term>Enzyme Induction</term><term>Enzyme Stability</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Basidiomycota</term><term>Induction enzymatique</term><term>Stabilité enzymatique</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN fongique</term><term>ARN messager</term><term>ARN ribosomique 16S</term><term>Basidiomycota</term><term>Endopeptidases</term><term>Isoenzymes</term><term>Lignine</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Alcools benzyliques</term><term>Lignine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phanerochaete chrysosporium is a white rot fungus which secretes a family of lignin-degrading enzymes under nutrient limitation. In this work, we investigated the roles of veratryl alcohol and lignin in the ligninolytic system of P. chrysosporium BKM-F-1767 cultures grown under nitrogen-limited conditions. Cultures supplemented with 0.4 to 2 mM veratryl alcohol showed increased lignin peroxidase activity. Addition of veratryl alcohol had no effect on Mn-dependent peroxidase activity and inhibited glyoxal oxidase activity. Azure-casein analysis of acidic proteases in the extracellular fluid showed that protease activity decreased during the early stages of secondary metabolism while lignin peroxidase activity was at its peak, suggesting that proteolysis was not involved in the regulation of lignin peroxidase activity during early secondary metabolism. In cultures supplemented with lignin or veratryl alcohol, no induction of mRNA coding for lignin peroxidase H2 or H8 was observed. Veratryl alcohol protected lignin peroxidase isozymes H2 and H8 from inactivation by H2O2. We conclude that veratryl alcohol acts as a stabilizer of lignin peroxidase activity and not as an inducer of lignin peroxidase synthesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8215363</PMID><DateCompleted><Year>1993</Year><Month>11</Month><Day>10</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0099-2240</ISSN><JournalIssue CitedMedium="Print"><Volume>59</Volume><Issue>9</Issue><PubDate><Year>1993</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Lignin and veratryl alcohol are not inducers of the ligninolytic system of Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>2909-13</MedlinePgn></Pagination><Abstract><AbstractText>Phanerochaete chrysosporium is a white rot fungus which secretes a family of lignin-degrading enzymes under nutrient limitation. In this work, we investigated the roles of veratryl alcohol and lignin in the ligninolytic system of P. chrysosporium BKM-F-1767 cultures grown under nitrogen-limited conditions. Cultures supplemented with 0.4 to 2 mM veratryl alcohol showed increased lignin peroxidase activity. Addition of veratryl alcohol had no effect on Mn-dependent peroxidase activity and inhibited glyoxal oxidase activity. Azure-casein analysis of acidic proteases in the extracellular fluid showed that protease activity decreased during the early stages of secondary metabolism while lignin peroxidase activity was at its peak, suggesting that proteolysis was not involved in the regulation of lignin peroxidase activity during early secondary metabolism. In cultures supplemented with lignin or veratryl alcohol, no induction of mRNA coding for lignin peroxidase H2 or H8 was observed. Veratryl alcohol protected lignin peroxidase isozymes H2 and H8 from inactivation by H2O2. We conclude that veratryl alcohol acts as a stabilizer of lignin peroxidase activity and not as an inducer of lignin peroxidase synthesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cancel</LastName><ForeName>A M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Orth</LastName><ForeName>A B</ForeName><Initials>AB</Initials></Author><Author ValidYN="Y"><LastName>Tien</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1-F32-ES05503-01</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>1-P42ES04922-01</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United 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MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1993</Year><Month>9</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1993</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1993</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8215363</ArticleId><ArticleId IdType="pmc">PMC182385</ArticleId><ArticleId IdType="doi">10.1128/AEM.59.9.2909-2913.1993</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 1991 Apr 15;176(1):269-75</Citation><ArticleIdList><ArticleId IdType="pubmed">2018522</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5</Citation><ArticleIdList><ArticleId IdType="pubmed">6326095</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1983 Aug 12;221(4611):661-3</Citation><ArticleIdList><ArticleId IdType="pubmed">17787736</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1985 Feb;49(2):299-304</Citation><ArticleIdList><ArticleId IdType="pubmed">16346716</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1990 Feb;56(2):395-400</Citation><ArticleIdList><ArticleId IdType="pubmed">16348114</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1988 Feb;54(2):466-72</Citation><ArticleIdList><ArticleId IdType="pubmed">16347560</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1985 Jul 15;260(14):8348-53</Citation><ArticleIdList><ArticleId IdType="pubmed">2989288</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Microbiol. 1978 Jun 26;117(3):227-38</Citation><ArticleIdList><ArticleId IdType="pubmed">100069</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1986 Aug;52(2):251-4</Citation><ArticleIdList><ArticleId IdType="pubmed">16347125</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1991 Sep;57(9):2591-6</Citation><ArticleIdList><ArticleId IdType="pubmed">1768132</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Microbiol. 1987;41:465-505</Citation><ArticleIdList><ArticleId IdType="pubmed">3318677</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1951 Nov;193(1):265-75</Citation><ArticleIdList><ArticleId IdType="pubmed">14907713</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1986 May 25;261(15):6900-3</Citation><ArticleIdList><ArticleId IdType="pubmed">3700421</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 1991 Aug 15;178(3):1056-63</Citation><ArticleIdList><ArticleId IdType="pubmed">1872828</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1990 Sep 18;29(37):8535-9</Citation><ArticleIdList><ArticleId IdType="pubmed">2271536</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1989 Aug 15;264(23):13531-5</Citation><ArticleIdList><ArticleId IdType="pubmed">2760033</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 1986 May 15;236(1):279-87</Citation><ArticleIdList><ArticleId IdType="pubmed">3024619</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>University Park (Pennsylvanie)</li></settlement><orgName><li>Université d'État de Pennsylvanie</li></orgName></list><tree><noCountry><name sortKey="Orth, A B" sort="Orth, A B" uniqKey="Orth A" first="A B" last="Orth">A B Orth</name><name sortKey="Tien, M" sort="Tien, M" uniqKey="Tien M" first="M" last="Tien">M. Tien</name></noCountry><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Cancel, A M" sort="Cancel, A M" uniqKey="Cancel A" first="A M" last="Cancel">A M Cancel</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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